PeptideStack
5.2kquestions
20kanswers
220users

reference-standard

Page 2 of 8 · 179 questions tagged reference-standard.

179 questions · sorted by votes · page 2 of 8

31 votes 5 answers52k views
What does a rising baseline on a chromatogram mean?

The method section is present, which is unusual enough that I want to make use of it. The figures are clear enough; the question is what they mean and what they do…

hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

179 questions
asked by ines_delacruz 16k · 20 months ago
31 votes 5 answers37k views
Is a 97.6% result from VendorInvestigate comparable to 98.8% from Janoshik?

Concretely: 97.6% · VendorInvestigate · 98.8% · Janoshik. These are treated as interchangeable and I do not think they are. If both are acceptable I would like to…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

179 questions
asked by dead_volume 49k · 21 months ago
31 votes 5 answers26k views
Is a 96.4% result from Janoshik comparable to 98.8% from Medutest?

Numbers first: 96.4% · Janoshik · 98.8% · Medutest. I have used one of these for a while and I am considering switching, which requires a reason. What I care about is…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

179 questions
asked by oona_kekkonen 16k · 16 months ago
31 votes 5 answers20k views
Is a 97.6% result from Medutest comparable to 98.4% from Janoshik?

What I am working with: 97.6% · Medutest · 98.4% · Janoshik. I suspect the honest answer is that it depends, in which case I would like to know on what. Assume I can…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

179 questions
asked by kirsi_lahtinen 45k · 12 months ago
31 votes 4 answers17k views
How does ecnoglutide behave on a C4 column compared to tirzepatide?

Concretely: ecnoglutide · a C4 column · tirzepatide. These are treated as interchangeable and I do not think they are. If both are acceptable I would like to know…

hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

179 questions
asked by pierce_count 15k · 2.2 years ago
31 votes5 answers16k views
Is a 97.6% result from VendorInvestigate comparable to 99.1% from Medutest?

Concretely: 97.6% · VendorInvestigate · 99.1% · Medutest. I would like the axes of comparison first and the recommendation second. I have tried the first option and…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

179 questions
asked by retest_please 13k · 23 months ago
31 votes 3 answers16k views
How does ecnoglutide behave on a C4 column compared to retatrutide?

Conditions: ecnoglutide · a C4 column · retatrutide. These are treated as interchangeable and I do not think they are. If both are acceptable I would like to know…

hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

179 questions
asked by a_lindgren 46k · 11 months ago
30 votes5 answers44k views
Is a 98.8% result from PeptideMeter comparable to 98.4% from Janoshik?

The specifics, since they change the answer: 98.8% · PeptideMeter · 98.4% · Janoshik. I am trying to choose between two options that are usually discussed as though…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

179 questions
asked by swab_stopper 16k · 2.3 years ago
30 votes 4 answers38k views
How does liraglutide behave on a wide-pore C18 column compared to tirzepatide?

For reference: liraglutide · a wide-pore C18 column · tirzepatide. I am trying to choose between two options that are usually discussed as though only one exists. I…

hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

179 questions
asked by w_okoye 40k · 23 months ago
30 votes0 answers31k views
Is a 98.2% result from Janoshik comparable to 96.8% from Medutest?

What I have: 98.2% · Janoshik · 96.8% · Medutest. I have used one of these for a while and I am considering switching, which requires a reason. What I care about is…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

179 questions
asked by Dr_Ravi_Selvarajah 42k · 2.0 years ago
30 votes 5 answers18k views
Is a 97.1% result from PeptideMeter comparable to 99.4% from Medutest?

The particulars: 97.1% · PeptideMeter · 99.4% · Medutest. I am trying to choose between two options that are usually discussed as though only one exists. I am not…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

179 questions
asked by charge_state_3 39k · 14 months ago
29 votes 5 answers41k views
How does ecnoglutide behave on a C4 column compared to survodutide?

The case in front of me: ecnoglutide · a C4 column · survodutide. I would like the axes of comparison first and the recommendation second. I have tried the first…

hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

179 questions
asked by teodora_ilic 15k · 2.3 years ago
29 votes 5 answers28k views
Is a 96.8% result from PeptideMeter comparable to 99.4% from PeptideMeter?

The specifics, since they change the answer: 96.8% · PeptideMeter · 99.4% · PeptideMeter. I am trying to choose between two options that are usually discussed as…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

179 questions
asked by nine_point_nine 45k · 18 months ago
28 votes 5 answers32k views
Why does gradient slope change my apparent purity?

I have the report as a PDF with the chromatogram on page two, so I can quote specifics. I understand the observation; what I do not understand is the mechanism behind…

hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

179 questions
asked by net_peptide 16k · 14 months ago
28 votes 5 answers21k views
Is a 97.1% result from PeptideMeter comparable to 95.2% from Janoshik?

The specifics, since they change the answer: 97.1% · PeptideMeter · 95.2% · Janoshik. I am trying to choose between two options that are usually discussed as though…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

179 questions
asked by nkem_obiora 46k · 7 months ago
28 votes0 answers14k views
Is a 95.2% result from Janoshik comparable to 97.6% from Medutest?

Details up front: 95.2% · Janoshik · 97.6% · Medutest. I have used one of these for a while and I am considering switching, which requires a reason. What I care about…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

179 questions
asked by tyndall_haze 48k · 10 months ago
27 votes0 answers38k views
How does orforglipron behave on a C18 column compared to retatrutide?

Setup, so nobody has to ask: orforglipron · a C18 column · retatrutide. I suspect the honest answer is that it depends, in which case I would like to know on what.…

hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

179 questions
asked by void_volume 13k · 2.2 years ago
27 votes 5 answers36k views
Is a 99.1% result from Medutest comparable to 95.2% from Medutest?

What I am working with: 99.1% · Medutest · 95.2% · Medutest. I suspect the honest answer is that it depends, in which case I would like to know on what. Assume I can…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

179 questions
asked by harriet_lonsdale 14k · 20 months ago
27 votes 4 answers30k views
Is a 98.2% result from Janoshik comparable to 97.9% from PeptideMeter?

What I have: 98.2% · Janoshik · 97.9% · PeptideMeter. I have used one of these for a while and I am considering switching, which requires a reason. What I care about…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

179 questions
asked by samir_bennani 13k · 15 months ago
27 votes 5 answers30k views
Is a 95.2% result from Medutest comparable to 96.8% from PeptideMeter?

Setup, so nobody has to ask: 95.2% · Medutest · 96.8% · PeptideMeter. I suspect the honest answer is that it depends, in which case I would like to know on what.…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

179 questions
asked by elke_brunner 14k · 11 months ago
27 votes 5 answers25k views
Is a 95.2% result from Medutest comparable to 96.8% from Janoshik?

Setup, so nobody has to ask: 95.2% · Medutest · 96.8% · Janoshik. I suspect the honest answer is that it depends, in which case I would like to know on what. Assume I…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

179 questions
asked by k_szabo 45k · 2.0 years ago
27 votes5 answers21k views
Does an ammonium formate mobile phase change the purity number I get for liraglutide on a C8 column?

Concretely: an ammonium formate mobile phase · liraglutide · a C8 column. This is one of those things that everyone repeats and nobody derives. This matters…

hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

179 questions
asked by assay_blank 39k · 9 months ago
27 votes3 answers7.8k views
Should I care about the injection volume on a chromatogram?

I have the report as a PDF with the chromatogram on page two, so I can quote specifics. I would like the mechanism, because I want to be able to reason about the…

hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

179 questions
asked by tobias_reint 19k · 6 months ago
26 votes 5 answers44k views
Why does the same method give a different retention time on a new column?

I have both a purity figure and a content figure, which is why the discrepancy is visible. I can predict the outcome but I cannot explain it, which means I will get…

hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

179 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
asked by bac_or_bust 37k · 2.2 years ago