Page 6 of 20 · 466 questions tagged hplc.
Setup, so nobody has to ask: 98.2% · a C18 column · ecnoglutide. I would like help reading this properly rather than being told what conclusion to reach. I have the…
The case in front of me: 99.1% · a C4 column · retatrutide. I would like to know the limits of what can be inferred from this. What I am trying to avoid is…
Concretely: an ammonium formate mobile phase · liraglutide · a C8 column. This is one of those things that everyone repeats and nobody derives. This matters…
The case in front of me: 96.8% · a C4 column · orforglipron. The figures are clear enough; the question is what they mean and what they do not. I can supply the…
Conditions: PeptideMeter · 97.6%. I think I have a problem. I am not yet sure whether it is a real problem or a measurement artefact. I want to know whether this is…
Details up front: 97.6% · dulaglutide. This should be a straightforward calculation and I keep getting two different answers. The numbers are arbitrary; the method is…
The particulars: liraglutide · 99.1%. I would rather understand the derivation than memorise the outcome. Two people I asked gave two answers that differ by a factor…
Numbers first: racemisation · orforglipron · minus 20 °C. I suspect the usual explanation for this is wrong, or at least incomplete. I am aware this may have a boring…
I have the report as a PDF with the chromatogram on page two, so I can quote specifics. I would like the mechanism, because I want to be able to reason about the…
I have both a purity figure and a content figure, which is why the discrepancy is visible. I can predict the outcome but I cannot explain it, which means I will get…
The specifics, since they change the answer: 99.1% · a wide-pore C18 column · mazdutide. I have the document in front of me and I can read the numbers. What I cannot…
For reference: 97.9% · ecnoglutide. I would like the arithmetic checked rather than the conclusion asserted. I have deliberately not used an online calculator because…
Setup, so nobody has to ask: Janoshik · 95.2%. This is not behaving the way I expected and I want to understand the discrepancy before I act on it. I have…
Numbers first: a GLP-1 receptor agonist · 96.4%. This should be a straightforward calculation and I keep getting two different answers. The numbers are arbitrary; the…
The particulars: 95.2% · a wide-pore C18 column · mazdutide. I have the document in front of me and I can read the numbers. What I cannot do is interpret them. I am…
Details up front: mazdutide · a C8 column · survodutide. I have used one of these for a while and I am considering switching, which requires a reason. What I care…
The case in front of me: 95.2% · a C4 column · retatrutide. The figures are clear enough; the question is what they mean and what they do not. I can supply the…
What I have: cagrilintide · 95.2%. I can do the algebra. I am not confident about the conversion factors. If there is a standard way to lay this out, I would rather…
The specifics, since they change the answer: liraglutide · a wide-pore C18 column · survodutide. I am trying to choose between two options that are usually discussed…
For reference: VendorInvestigate · 99.1%. Something has gone wrong and I would like to know how badly before I decide what to do. Nothing else in the setup changed,…
Stated plainly: 97.1% · a C18 column · ecnoglutide. I would like help reading this properly rather than being told what conclusion to reach. I have the full report…
The specifics, since they change the answer: deamidation · semaglutide · room temperature. I would like the mechanism, because I want to be able to reason about the…
Setup, so nobody has to ask: 99.4% · a C18 column · ecnoglutide. I have read the primary source rather than the summary, which has left me with more questions. I…
The specifics, since they change the answer: fibrillation · semaglutide · 40 °C. I understand the observation; what I do not understand is the mechanism behind it. I…