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purity

Page 3 of 33 · 788 questions tagged purity.

788 questions · sorted by votes · page 3 of 33

36 votes5 answers45k views
Would you accept 95.2% on liraglutide from ERP without a content assay?

Concretely: 95.2% · liraglutide · ERP. I want to decide this in advance so that I am not deciding it under pressure later. Assume I will follow the plan I write down,…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

438 questions
vendor-vetting
vendor-vetting

Evaluating a supplier on evidence rather than reputation: testing history across batches, whether certificates are batch-specific, how failures…

436 questions
asked by oona_kekkonen 16k · 23 months ago
36 votes 5 answers34k views
How do I reconcile 99.1% from VendorInvestigate with 99.4% from the supplier?

The case in front of me: 99.1% · VendorInvestigate · 99.4%. I think I have a problem. I am not yet sure whether it is a real problem or a measurement artefact. I want…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

749 questions
asked by leah_ferrers 15k · 2.1 years ago
36 votes 5 answers34k views
Is a 96.4% result from VendorInvestigate comparable to 99.4% from Janoshik?

Concretely: 96.4% · VendorInvestigate · 99.4% · Janoshik. I would like the axes of comparison first and the recommendation second. I have tried the first option and…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

179 questions
asked by s_bhattacharya 42k · 2.1 years ago
36 votes5 answers29k views
How does retatrutide behave on a C18 column compared to retatrutide?

What I am working with: retatrutide · a C18 column · retatrutide. Both of these get recommended confidently by different people, which suggests neither is obviously…

hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

179 questions
asked by Dr_Ingrid_Baumgartner 39k · 2.2 years ago
36 votes 3 answers22k views
What counter-ion should I expect on an SGN dulaglutide lot?

Numbers first: SGN · dulaglutide. I can parse the result. I am less sure what it licenses me to conclude. I have deliberately not looked at anyone else’s…

acetate-content
acetate-content

Counter-ion content, most often acetate or trifluoroacetate, and why it matters: the counter-ion and residual water are part of the gross mass you…

87 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

438 questions
asked by syringe_ninety 15k · 17 months ago
36 votes0 answers22k views
Is a 97.6% result from VendorInvestigate comparable to 96.8% from PeptideMeter?

The case in front of me: 97.6% · VendorInvestigate · 96.8% · PeptideMeter. These are treated as interchangeable and I do not think they are. If both are acceptable I…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

179 questions
asked by ruaidhri_o_shea 51k · 2.1 years ago
36 votes 5 answers19k views
Does dimerisation of mazdutide at room temperature show up as a loss of content or of purity?

Stated plainly: dimerisation · mazdutide · room temperature. I keep seeing this stated as a fact with no explanation attached, and unexplained facts make me…

peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

438 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
asked by label_claim 11k · 18 months ago
35 votes 5 answers77k views
Would you accept 97.9% on ecnoglutide from JEEP without a content assay?

For reference: 97.9% · ecnoglutide · JEEP. I am at the decision point and I would rather think it through than improvise. I would rather spend money on measurement…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

438 questions
vendor-vetting
vendor-vetting

Evaluating a supplier on evidence rather than reputation: testing history across batches, whether certificates are batch-specific, how failures…

436 questions
asked by seven_day_half 16k · 2.3 years ago
35 votes 5 answers65k views
What does identity confirmation by mass spectrometry add for ecnoglutide that a purity figure does not?

The particulars: identity confirmation by mass spectrometry · ecnoglutide. I am trying to choose between two options that are usually discussed as though only one…

content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

438 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

749 questions
asked by pieter_maas 22k · 2.3 years ago
35 votes4 answers64k views
What does a 97.1% purity with a lot-specific certificate attached let me conclude?

What I have: 97.1% · lot-specific certificate. This is presented as though it settles something, and I am not convinced it does. I have two documents that appear to…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

749 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
asked by mg_per_ml 12k · 2.1 years ago
35 votes 2 answers63k views
How do I reconcile 96.4% from PeptideMeter with 98.8% from the supplier?

The particulars: 96.4% · PeptideMeter · 98.8%. An unexpected observation, and I would like a differential rather than reassurance. The conditions were within what I…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

749 questions
asked by sample_id 17k · 2.2 years ago
35 votes 5 answers58k views
What counter-ion should I expect on an SSA ecnoglutide lot?

The particulars: SSA · ecnoglutide. I want to understand what this actually establishes, as opposed to what it is being used to imply. My concern is that I am being…

acetate-content
acetate-content

Counter-ion content, most often acetate or trifluoroacetate, and why it matters: the counter-ion and residual water are part of the gross mass you…

87 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

438 questions
asked by g_paskevicius 44k · 2.4 years ago
35 votes 5 answers47k views
Is a 95.2% result from PeptideMeter comparable to 98.8% from Janoshik?

The particulars: 95.2% · PeptideMeter · 98.8% · Janoshik. The comparison I want does not seem to exist anywhere in a form I can evaluate. I have read the arguments…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

179 questions
asked by rae_oyelowo 21k · 2.0 years ago
35 votes 5 answers42k views
Is a 99.4% result from Janoshik comparable to 97.1% from Janoshik?

Details up front: 99.4% · Janoshik · 97.1% · Janoshik. I have used one of these for a while and I am considering switching, which requires a reason. What I care about…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

179 questions
asked by bounty_hunter_q 18k · 22 months ago
35 votes 4 answers42k views
How do I reconcile 98.8% from PeptideMeter with 97.9% from the supplier?

The specifics, since they change the answer: 98.8% · PeptideMeter · 97.9%. An unexpected observation, and I would like a differential rather than reassurance. The…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

749 questions
asked by net_peptide 16k · 22 months ago
35 votes 5 answers41k views
Would you accept 98.8% on oral semaglutide from QSC without a content assay?

The case in front of me: 98.8% · oral semaglutide · QSC. The failure mode I am trying to avoid is making this decision emotionally. I have twelve months in view and I…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

438 questions
vendor-vetting
vendor-vetting

Evaluating a supplier on evidence rather than reputation: testing history across batches, whether certificates are batch-specific, how failures…

436 questions
asked by gunnar_isaksen 16k · 14 months ago
35 votes0 answers37k views
What does a 98.4% purity with a chromatogram attached let me conclude?

The specifics, since they change the answer: 98.4% · chromatogram. I want to understand what this actually establishes, as opposed to what it is being used to imply.…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

749 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
asked by fill_volume 13k · 22 months ago
35 votes 5 answers34k views
What does 98.8% purity on a wide-pore C18 column actually tell me about a GLP-1 receptor agonist vial?

The particulars: 98.8% · a wide-pore C18 column · a GLP-1 receptor agonist. I want to understand what this actually establishes, as opposed to what it is being used…

hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

749 questions
asked by pk_curve 12k · 2.3 years ago
35 votes5 answers31k views
How do I compute the +2 charge state m/z for a peptide of 4207.2 Da?

What I have: +2 · 4207.2 Da. This should be a straightforward calculation and I keep getting two different answers. The numbers are arbitrary; the method is what I am…

mass-spec
mass-spec

Mass spectrometry for identity confirmation: electrospray ionisation, multiple charge states, monoisotopic versus average mass, deconvolution, and…

38 questions
dosing-math
dosing-math

The arithmetic itself: milligrams to millilitres to insulin units, concentration after reconstitution, dose per draw, and vial-days per vial. Show…

811 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
asked by liam_bracken 12k · 22 months ago
35 votes 5 answers29k views
How do I reconcile 99.4% from PeptideMeter with 97.1% from the supplier?

The particulars: 99.4% · PeptideMeter · 97.1%. Something has gone wrong and I would like to know how badly before I decide what to do. Nothing else in the setup…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

749 questions
asked by Dr_Rosalind_Achebe 90k · 20 months ago
35 votes0 answers28k views
Would you accept 99.4% on retatrutide from GL Biochem without a content assay?

Details up front: 99.4% · retatrutide · GL Biochem. I would rather over-plan the first cycle and simplify later. I am prepared to do the work if someone can tell me…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

438 questions
vendor-vetting
vendor-vetting

Evaluating a supplier on evidence rather than reputation: testing history across batches, whether certificates are batch-specific, how failures…

436 questions
asked by tare_and_weigh 18k · 23 months ago
35 votes 5 answers22k views
Can I trust a purity figure quoted without a chromatogram?

I have the report as a PDF with the chromatogram on page two, so I can quote specifics. Somebody stated this to me confidently and I would like to check it before…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

749 questions
vendor-vetting
vendor-vetting

Evaluating a supplier on evidence rather than reputation: testing history across batches, whether certificates are batch-specific, how failures…

436 questions
asked by nils_karlberg 13k · 2.1 years ago
35 votes 3 answers16k views
Two labs reported 98.1% and 99.4% on the same vial. Which one is wrong?

I split one vial and sent halves to two independent laboratories. Purity came back 98.1% and 99.4%. Both sent chromatograms. Both look competent. The 1.3-point gap is…

hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

749 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
janoshik
janoshik

Janoshik Analytical, an independent third-party testing laboratory widely used by this community for HPLC purity, identity and quantified content…

34 questions
asked by noor_alhassan 15k · 12 months ago
34 votes5 answers56k views
Why would Janoshik report 97.6% where the supplier certificate says something higher?

Setup, so nobody has to ask: Janoshik · 97.6%. I noticed this today and I have not touched anything since, in case the state is diagnostic. I have not discarded…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
asked by cold_lane 14k · 23 months ago