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peptide-mapping

Page 4 of 4 · 83 questions tagged peptide-mapping.

83 questions · sorted by votes · page 4 of 4

7 votes 5 answers5.4k views
Would a quantified content assay distinguish fibrillation from a synthesis impurity in dulaglutide?

The case in front of me: a quantified content assay · fibrillation · dulaglutide. These are treated as interchangeable and I do not think they are. If both are…

peptide-mapping
peptide-mapping

Enzymatic digestion followed by LC-MS/MS to confirm sequence rather than just mass. The method that catches a scrambled sequence or a D-amino-acid…

83 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
mass-spec
mass-spec

Mass spectrometry for identity confirmation: electrospray ionisation, multiple charge states, monoisotopic versus average mass, deconvolution, and…

38 questions
asked by Dr_Fatima_Belkacem 52k · 14 months ago
6 votes5 answers15k views
Is 96.8% a realistic purity ceiling for retatrutide given its chain length?

Details up front: 96.8% · retatrutide. This should be a straightforward calculation and I keep getting two different answers. The numbers are arbitrary; the method is…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
peptide-mapping
peptide-mapping

Enzymatic digestion followed by LC-MS/MS to confirm sequence rather than just mass. The method that catches a scrambled sequence or a D-amino-acid…

83 questions
asked by sinead_gaffney 14k · 2.2 years ago
6 votes5 answers9.5k views
What does peptide mapping catch that mass spectrometry alone misses?

I have the report as a PDF with the chromatogram on page two, so I can quote specifics. The comparison I want does not seem to exist anywhere in a form I can…

peptide-mapping
peptide-mapping

Enzymatic digestion followed by LC-MS/MS to confirm sequence rather than just mass. The method that catches a scrambled sequence or a D-amino-acid…

83 questions
mass-spec
mass-spec

Mass spectrometry for identity confirmation: electrospray ionisation, multiple charge states, monoisotopic versus average mass, deconvolution, and…

38 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
asked by hana_petrikova 19k · 6 months ago
6 votes0 answers3k views
Is 98.8% a realistic purity ceiling for orforglipron given its chain length?

Numbers first: 98.8% · orforglipron. I can do the algebra. I am not confident about the conversion factors. If there is a standard way to lay this out, I would rather…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
peptide-mapping
peptide-mapping

Enzymatic digestion followed by LC-MS/MS to confirm sequence rather than just mass. The method that catches a scrambled sequence or a D-amino-acid…

83 questions
asked by imani_dube 19k · 18 months ago
5 votes5 answers19k views
Is 99.4% a realistic purity ceiling for cagrilintide given its chain length?

Stated plainly: 99.4% · cagrilintide. The units are where I keep going wrong, so please be explicit about them. I have sanity-checked the order of magnitude and it…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
peptide-mapping
peptide-mapping

Enzymatic digestion followed by LC-MS/MS to confirm sequence rather than just mass. The method that catches a scrambled sequence or a D-amino-acid…

83 questions
asked by cal_hennessy 14k · 2.3 years ago
5 votes0 answers3.2k views
Is 97.1% a realistic purity ceiling for tirzepatide given its chain length?

Conditions: 97.1% · tirzepatide. Please show the division. I want to check my own against yours. I would like the general form as well as the specific number, so I…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
peptide-mapping
peptide-mapping

Enzymatic digestion followed by LC-MS/MS to confirm sequence rather than just mass. The method that catches a scrambled sequence or a D-amino-acid…

83 questions
asked by rota_site 55k · 18 months ago
4 votes 2 answers3.1k views
Is 98.8% a realistic purity ceiling for liraglutide given its chain length?

Conditions: 98.8% · liraglutide. Please show the division. I want to check my own against yours. I would like the general form as well as the specific number, so I…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
peptide-mapping
peptide-mapping

Enzymatic digestion followed by LC-MS/MS to confirm sequence rather than just mass. The method that catches a scrambled sequence or a D-amino-acid…

83 questions
asked by retest_please 13k · 12 months ago
3 votes5 answers9.9k views
Is 99.4% a realistic purity ceiling for ecnoglutide given its chain length?

The particulars: 99.4% · ecnoglutide. I would rather understand the derivation than memorise the outcome. Two people I asked gave two answers that differ by a factor…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
peptide-mapping
peptide-mapping

Enzymatic digestion followed by LC-MS/MS to confirm sequence rather than just mass. The method that catches a scrambled sequence or a D-amino-acid…

83 questions
asked by dead_volume 49k · 10 months ago
2 votes 5 answers19k views
Is 97.1% a realistic purity ceiling for liraglutide given its chain length?

Concretely: 97.1% · liraglutide. I want the working, not the result — I need to be able to redo it with different numbers. I care about the precision as well as the…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
peptide-mapping
peptide-mapping

Enzymatic digestion followed by LC-MS/MS to confirm sequence rather than just mass. The method that catches a scrambled sequence or a D-amino-acid…

83 questions
asked by thermal_mass 16k · 2.3 years ago
1 vote 4 answers5.7k views
Is 98.4% a realistic purity ceiling for a GLP-1 receptor agonist given its chain length?

What I am working with: 98.4% · a GLP-1 receptor agonist. I have worked this out and I would like someone to find the error, because I suspect there is one. My…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
peptide-mapping
peptide-mapping

Enzymatic digestion followed by LC-MS/MS to confirm sequence rather than just mass. The method that catches a scrambled sequence or a D-amino-acid…

83 questions
asked by Dr_Ravi_Selvarajah 42k · 9 months ago
1 vote0 answers1.4k views
Would a Karl Fischer water content test distinguish deamidation from a synthesis impurity in dulaglutide?

Conditions: a Karl Fischer water content test · deamidation · dulaglutide. I would like the axes of comparison first and the recommendation second. I have tried the…

peptide-mapping
peptide-mapping

Enzymatic digestion followed by LC-MS/MS to confirm sequence rather than just mass. The method that catches a scrambled sequence or a D-amino-acid…

83 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
mass-spec
mass-spec

Mass spectrometry for identity confirmation: electrospray ionisation, multiple charge states, monoisotopic versus average mass, deconvolution, and…

38 questions
asked by mira_sundqvist 19k · 10 months ago