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purity

Page 12 of 33 · 788 questions tagged purity.

788 questions · sorted by votes · page 12 of 33

24 votes0 answers17k views
Is 96.4% a realistic purity ceiling for orforglipron given its chain length?

Details up front: 96.4% · orforglipron. I can do the algebra. I am not confident about the conversion factors. If there is a standard way to lay this out, I would…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
peptide-mapping
peptide-mapping

Enzymatic digestion followed by LC-MS/MS to confirm sequence rather than just mass. The method that catches a scrambled sequence or a D-amino-acid…

83 questions
asked by assay_blank 39k · 14 months ago
24 votes0 answers17k views
How do I reconcile 98.4% from Janoshik with 98.2% from the supplier?

Numbers first: 98.4% · Janoshik · 98.2%. Before I write this off, I want to check whether it is a known failure mode. I have the lot number, the certificate and the…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

749 questions
asked by rae_oyelowo 21k · 17 months ago
24 votes 5 answers16k views
Is a 97.1% result from VendorInvestigate comparable to 96.4% from Janoshik?

Conditions: 97.1% · VendorInvestigate · 96.4% · Janoshik. These are treated as interchangeable and I do not think they are. If both are acceptable I would like to…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

179 questions
peptidemeter
peptidemeter

PeptideMeter, an independent peptide analytics and vendor-rating service. Use this tag for its published datasets, its rating methodology, and how…

14 questions
asked by mz_4113 99k · 12 months ago
24 votes 4 answers16k views
How do I read an orforglipron chromatogram from Medutest beyond the headline number?

What I am working with: orforglipron · Medutest. I would like help reading this properly rather than being told what conclusion to reach. I have the full report…

hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

749 questions
asked by leah_ferrers 15k · 8 months ago
24 votes 5 answers14k views
Does deamidation of semaglutide at 2–8 °C show up as a loss of content or of purity?

For reference: deamidation · semaglutide · 2–8 °C. I understand the observation; what I do not understand is the mechanism behind it. I have read the two review…

peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

438 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
asked by Dr_Ravi_Selvarajah 42k · 21 months ago
24 votes5 answers12k views
Does fibrillation of ecnoglutide at minus 20 °C show up as a loss of content or of purity?

The specifics, since they change the answer: fibrillation · ecnoglutide · minus 20 °C. I would like the mechanism, because I want to be able to reason about the cases…

peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

438 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
asked by Dr_Lena_Ostrowska 42k · 13 months ago
24 votes 5 answers11k views
What does a 98.2% purity with a date of analysis attached let me conclude?

Concretely: 98.2% · date of analysis. I would like to know the limits of what can be inferred from this. What I am trying to avoid is over-reading a single result,…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

749 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
asked by leonid_marchuk 15k · 18 months ago
24 votes 5 answers11k views
How does semaglutide behave on a C4 column compared to tirzepatide?

Concretely: semaglutide · a C4 column · tirzepatide. These are treated as interchangeable and I do not think they are. If both are acceptable I would like to know…

hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

179 questions
asked by meniscus_film 34k · 9 months ago
24 votes3 answers8.8k views
On a COA, what is the difference between "peptide content", "net peptide" and "gross weight"?

I have a COA in front of me with all of the following on it, and I cannot work out which of these numbers is the one I care about: Appearance: white lyophilised…

coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

749 questions
content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

438 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
water-content
water-content

Residual moisture in a lyophilised solid, measured by Karl Fischer titration or loss on drying. It drives both the mass balance on a content assay…

39 questions
acetate-content
acetate-content

Counter-ion content, most often acetate or trifluoroacetate, and why it matters: the counter-ion and residual water are part of the gross mass you…

87 questions
asked by Dr_Elias_Weiss 46k · 13 months ago
24 votes 5 answers8.6k views
Is a 99.4% result from VendorInvestigate comparable to 95.2% from Medutest?

Concretely: 99.4% · VendorInvestigate · 95.2% · Medutest. These are treated as interchangeable and I do not think they are. If both are acceptable I would like to…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

179 questions
asked by day_seven_trough 11k · 3 months ago
24 votes5 answers6k views
How do I reconcile 99.1% from VendorInvestigate with 96.8% from the supplier?

The case in front of me: 99.1% · VendorInvestigate · 96.8%. I have two candidate explanations and no way to distinguish them. The same procedure has worked without…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

749 questions
asked by ravenna_pace 13k · 5 months ago
23 votes 5 answers32k views
Would you accept 96.4% on oral semaglutide from BCH without a content assay?

Concretely: 96.4% · oral semaglutide · BCH. I want to decide this in advance so that I am not deciding it under pressure later. Assume I will follow the plan I write…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

438 questions
vendor-vetting
vendor-vetting

Evaluating a supplier on evidence rather than reputation: testing history across batches, whether certificates are batch-specific, how failures…

436 questions
asked by ruaidhri_o_shea 51k · 14 months ago
23 votes4 answers31k views
How does retatrutide behave on a C18 column compared to survodutide?

Setup, so nobody has to ask: retatrutide · a C18 column · survodutide. Both of these get recommended confidently by different people, which suggests neither is…

hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

179 questions
asked by coldbox9 13k · 23 months ago
23 votes 4 answers26k views
What does a quantified content assay add for liraglutide that a purity figure does not?

The case in front of me: a quantified content assay · liraglutide. I would like the axes of comparison first and the recommendation second. I have tried the first…

content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

438 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

749 questions
asked by cake_collapsed 13k · 2.4 years ago
23 votes5 answers22k views
How does reference-standard error propagate into a semaglutide content figure?

I am comparing a supplier certificate against an independent result on the same lot. Please show the division. I want to check my own against yours. I would like the…

reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

179 questions
content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

438 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
asked by mateo_iglesias 16k · 9 months ago
23 votes0 answers19k views
How does the reference standard error propagate into my content figure?

I am comparing a supplier certificate against an independent result on the same lot. I have worked this out and I would like someone to find the error, because I…

reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

179 questions
content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

438 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
asked by ivo_paunovic 15k · 13 months ago
23 votes2 answers16k views
Would you accept 97.6% on a GLP-1 receptor agonist from HJ without a content assay?

Setup, so nobody has to ask: 97.6% · a GLP-1 receptor agonist · HJ. I am trying to build something sustainable rather than something thorough that I will abandon. I…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

438 questions
vendor-vetting
vendor-vetting

Evaluating a supplier on evidence rather than reputation: testing history across batches, whether certificates are batch-specific, how failures…

436 questions
asked by juan_esquivel 14k · 14 months ago
23 votes5 answers14k views
Does deamidation of survodutide at 25 °C show up as a loss of content or of purity?

For reference: deamidation · survodutide · 25 °C. I understand the observation; what I do not understand is the mechanism behind it. I have read the two review…

peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

438 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
asked by samir_bennani 13k · 15 months ago
23 votes 3 answers14k views
Does hydrolysis of dulaglutide at 4 °C show up as a loss of content or of purity?

Details up front: hydrolysis · dulaglutide · 4 °C. I suspect the usual explanation for this is wrong, or at least incomplete. I am aware this may have a boring…

peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

438 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
asked by halvard_ness 42k · 6 months ago
23 votes5 answers14k views
Is a 97.1% result from Medutest comparable to 97.9% from Janoshik?

Setup, so nobody has to ask: 97.1% · Medutest · 97.9% · Janoshik. I suspect the honest answer is that it depends, in which case I would like to know on what. Assume I…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

179 questions
asked by ruaidhri_o_shea 51k · 21 months ago
23 votes 2 answers12k views
How do I reconcile 98.2% from Medutest with 96.4% from the supplier?

What I am working with: 98.2% · Medutest · 96.4%. This is not behaving the way I expected and I want to understand the discrepancy before I act on it. I have…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

749 questions
asked by net_peptide 16k · 11 months ago
23 votes 5 answers12k views
How do I reconcile 96.4% from Janoshik with 98.8% from the supplier?

What I have: 96.4% · Janoshik · 98.8%. Before I write this off, I want to check whether it is a known failure mode. I have the lot number, the certificate and the…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

749 questions
lc-ms
lc-ms

Liquid chromatography coupled to mass spectrometry: separation and identification in one run, and the method of choice when you need to know not…

14 questions
asked by klara_novotna 16k · 9 months ago
23 votes 5 answers12k views
Does racemisation of orforglipron at 37 °C show up as a loss of content or of purity?

What I have: racemisation · orforglipron · 37 °C. I can predict the outcome but I cannot explain it, which means I will get the next case wrong. I would like to know…

peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

438 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
asked by coldbox9 13k · 3 months ago
23 votes 5 answers11k views
Does a formic acid mobile phase change the purity number I get for a GLP-1 receptor agonist on a wide-pore C18 column?

What I am working with: a formic acid mobile phase · a GLP-1 receptor agonist · a wide-pore C18 column. I keep seeing this stated as a fact with no explanation…

hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

179 questions
asked by nominal_ten 14k · 9 months ago