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purity

Page 16 of 33 · 788 questions tagged purity.

788 questions · sorted by votes · page 16 of 33

20 votes 5 answers24k views
What does a 95.2% purity with a batch certificate attached let me conclude?

What I am working with: 95.2% · batch certificate. I have read the primary source rather than the summary, which has left me with more questions. I understand the…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

749 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
asked by leonid_marchuk 15k · 20 months ago
20 votes 5 answers24k views
Does aspartimide formation of tirzepatide at 37 °C show up as a loss of content or of purity?

Conditions: aspartimide formation · tirzepatide · 37 °C. This is one of those things that everyone repeats and nobody derives. This matters practically, not just…

peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

438 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
asked by anders_vestby 17k · 16 months ago
20 votes0 answers23k views
Why would Janoshik report 99.4% where the supplier certificate says something higher?

What I am working with: Janoshik · 99.4%. I noticed this today and I have not touched anything since, in case the state is diagnostic. I have not discarded anything…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
asked by esben_lykke 15k · 20 months ago
20 votes5 answers23k views
Does fibrillation of semaglutide at room temperature show up as a loss of content or of purity?

The particulars: fibrillation · semaglutide · room temperature. I would like the mechanism, because I want to be able to reason about the cases nobody has written…

peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

438 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
asked by hana_petrikova 19k · 11 months ago
20 votes0 answers22k views
Why does my content assay disagree with the label by more than purity would allow?

The method section is present, which is unusual enough that I want to make use of it. I noticed this today and I have not touched anything since, in case the state is…

content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

438 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

749 questions
asked by pieter_maas 22k · 2.2 years ago
20 votes 5 answers20k views
What counter-ion should I expect on a GL Biochem ecnoglutide lot?

The specifics, since they change the answer: GL Biochem · ecnoglutide. I want to understand what this actually establishes, as opposed to what it is being used to…

acetate-content
acetate-content

Counter-ion content, most often acetate or trifluoroacetate, and why it matters: the counter-ion and residual water are part of the gross mass you…

87 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

438 questions
asked by cake_intact 18k · 14 months ago
20 votes 5 answers17k views
Is 97.6% a realistic purity ceiling for tirzepatide given its chain length?

Conditions: 97.6% · tirzepatide. Please show the division. I want to check my own against yours. I would like the general form as well as the specific number, so I…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
peptide-mapping
peptide-mapping

Enzymatic digestion followed by LC-MS/MS to confirm sequence rather than just mass. The method that catches a scrambled sequence or a D-amino-acid…

83 questions
lc-ms
lc-ms

Liquid chromatography coupled to mass spectrometry: separation and identification in one run, and the method of choice when you need to know not…

14 questions
asked by ravenna_pace 13k · 7 months ago
20 votes 5 answers17k views
What does a 99.4% purity with a date of analysis attached let me conclude?

Concretely: 99.4% · date of analysis. I would like to know the limits of what can be inferred from this. What I am trying to avoid is over-reading a single result,…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

749 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
asked by rhian_prydderch 44k · 15 months ago
20 votes0 answers13k views
Why would Janoshik report 98.8% where the supplier certificate says something higher?

What I am working with: Janoshik · 98.8%. I noticed this today and I have not touched anything since, in case the state is diagnostic. I have not discarded anything…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
asked by ben_akintola 14k · 2.3 years ago
20 votes 5 answers11k views
Would you accept 96.4% on cagrilintide from CPC without a content assay?

What I am working with: 96.4% · cagrilintide · CPC. I am trying to build something sustainable rather than something thorough that I will abandon. I have already…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

438 questions
vendor-vetting
vendor-vetting

Evaluating a supplier on evidence rather than reputation: testing history across batches, whether certificates are batch-specific, how failures…

436 questions
asked by anja_hellstrom 13k · 3 months ago
20 votes 5 answers9.9k views
How do I reconcile 95.2% from Janoshik with 99.4% from the supplier?

What I have: 95.2% · Janoshik · 99.4%. I have a result I cannot explain, and I would rather diagnose it than guess. I have checked the obvious explanations and…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

749 questions
asked by cake_intact 18k · 16 months ago
20 votes5 answers8.9k views
Does oxidation of liraglutide at 30 °C show up as a loss of content or of purity?

Conditions: oxidation · liraglutide · 30 °C. I want to know whether this is a real physical effect or an artefact of how it is measured. What prompted the question is…

peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

438 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
asked by cap_the_luer 15k · 3 months ago
20 votes 5 answers8.4k views
How do I compute the +3 charge state m/z for a peptide of 4113.6 Da?

What I am working with: +3 · 4113.6 Da. I have worked this out and I would like someone to find the error, because I suspect there is one. My working so far, for the…

mass-spec
mass-spec

Mass spectrometry for identity confirmation: electrospray ionisation, multiple charge states, monoisotopic versus average mass, deconvolution, and…

38 questions
dosing-math
dosing-math

The arithmetic itself: milligrams to millilitres to insulin units, concentration after reconstitution, dose per draw, and vial-days per vial. Show…

811 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
asked by thabo_maseko 20k · 5 months ago
20 votes 3 answers8.2k views
Is 96.8% a realistic purity ceiling for orforglipron given its chain length?

Details up front: 96.8% · orforglipron. This should be a straightforward calculation and I keep getting two different answers. The numbers are arbitrary; the method…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
peptide-mapping
peptide-mapping

Enzymatic digestion followed by LC-MS/MS to confirm sequence rather than just mass. The method that catches a scrambled sequence or a D-amino-acid…

83 questions
asked by bridget_nyathi 16k · 35 days ago
20 votes 5 answers8.1k views
How do I reconcile 97.1% from Janoshik with 98.4% from the supplier?

What I have: 97.1% · Janoshik · 98.4%. Before I write this off, I want to check whether it is a known failure mode. I have the lot number, the certificate and the…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

749 questions
asked by second_lot 11k · 4 months ago
20 votes 5 answers5.9k views
What does a 97.1% purity with a list of related substances attached let me conclude?

The particulars: 97.1% · list of related substances. I have the document in front of me and I can read the numbers. What I cannot do is interpret them. I am…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

749 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
asked by klara_novotna 16k · 4 months ago
20 votes 4 answers5.5k views
C18 or C8, and TFA or formic acid or phosphate — how much does each choice move the purity figure?

I am trying to specify a method to a contract lab rather than accept their default, and I do not know enough to write a sensible specification. The choices I can see…

hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

179 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
tirzepatide
tirzepatide

A dual GIP and GLP-1 receptor agonist. Questions here cover the SURPASS and SURMOUNT programmes, the practical differences from a pure GLP-1…

162 questions
asked by elke_brunner 14k · 2 months ago
20 votes0 answers5.1k views
Would you accept 98.4% on tirzepatide from BCH without a content assay?

Concretely: 98.4% · tirzepatide · BCH. The failure mode I am trying to avoid is making this decision emotionally. I have twelve months in view and I would like the…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

438 questions
vendor-vetting
vendor-vetting

Evaluating a supplier on evidence rather than reputation: testing history across batches, whether certificates are batch-specific, how failures…

436 questions
asked by u100_marks 38k · 20 days ago
20 votes 5 answers4.8k views
What does a quantified content assay add for tirzepatide that a purity figure does not?

The case in front of me: a quantified content assay · tirzepatide. I would like the axes of comparison first and the recommendation second. I have tried the first…

content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

438 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

749 questions
asked by grainne_ahearn 13k · 3 months ago
20 votes5 answers3.8k views
Why would PeptideMeter report 99.1% where the supplier certificate says something higher?

Concretely: PeptideMeter · 99.1%. I think I have a problem. I am not yet sure whether it is a real problem or a measurement artefact. I want to know whether this is…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
asked by dana_wexler 15k · 40 days ago
20 votes 5 answers3.3k views
Is a 98.4% result from VendorInvestigate comparable to 97.6% from PeptideMeter?

The case in front of me: 98.4% · VendorInvestigate · 97.6% · PeptideMeter. I would like the axes of comparison first and the recommendation second. I have tried the…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

179 questions
asked by helena_vidmar 18k · 3 months ago
19 votes 5 answers25k views
What counter-ion should I expect on a GL Biochem survodutide lot?

The particulars: GL Biochem · survodutide. I want to understand what this actually establishes, as opposed to what it is being used to imply. My concern is that I am…

acetate-content
acetate-content

Counter-ion content, most often acetate or trifluoroacetate, and why it matters: the counter-ion and residual water are part of the gross mass you…

87 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

438 questions
asked by rosa_mendieta 13k · 2.1 years ago
19 votes 5 answers23k views
Why would VendorInvestigate report 96.8% where the supplier certificate says something higher?

For reference: VendorInvestigate · 96.8%. An unexpected observation, and I would like a differential rather than reassurance. The conditions were within what I…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
asked by e_dziedzic 87k · 14 months ago
19 votes 5 answers22k views
How do I compute the +2 charge state m/z for a peptide of 4113.6 Da?

Details up front: +2 · 4113.6 Da. I can do the algebra. I am not confident about the conversion factors. If there is a standard way to lay this out, I would rather…

mass-spec
mass-spec

Mass spectrometry for identity confirmation: electrospray ionisation, multiple charge states, monoisotopic versus average mass, deconvolution, and…

38 questions
dosing-math
dosing-math

The arithmetic itself: milligrams to millilitres to insulin units, concentration after reconstitution, dose per draw, and vial-days per vial. Show…

811 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
asked by rota_site 55k · 12 months ago