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freeze-thaw

Page 2 of 3 · 55 questions tagged freeze-thaw.

55 questions · sorted by votes · page 2 of 3

22 votes 2 answers15k views
How many freeze-thaw cycles will orforglipron at 5 mg/mL tolerate?

Stated plainly: orforglipron · 5 mg/mL. This is a procedural question rather than a theoretical one, and I would like the procedure rather than the theory. What I…

freeze-thaw
freeze-thaw

What repeated freezing does to a peptide in solution: ice-crystal shear at the growing front, freeze-concentration of solutes, pH shifts as buffer…

55 questions
peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
storage
storage

Storage conditions and their evidence base: minus twenty degrees for powder, refrigerated for solution, protection from light, and what the…

646 questions
asked by bac_or_bust 37k · 7 months ago
22 votes1 answer3.6k views
How many freeze-thaw cycles will liraglutide at 10 mg/mL tolerate?

For reference: liraglutide · 10 mg/mL. I have read the obvious sources and they disagree with each other, so I would rather ask people who have actually done this. I…

freeze-thaw
freeze-thaw

What repeated freezing does to a peptide in solution: ice-crystal shear at the growing front, freeze-concentration of solutes, pH shifts as buffer…

55 questions
peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
storage
storage

Storage conditions and their evidence base: minus twenty degrees for powder, refrigerated for solution, protection from light, and what the…

646 questions
asked by Dr_Tomas_Kral 37k · 25 days ago
21 votes 5 answers41k views
How many freeze-thaw cycles will tirzepatide at 10 mg/mL tolerate?

For reference: tirzepatide · 10 mg/mL. I have read the obvious sources and they disagree with each other, so I would rather ask people who have actually done this. I…

freeze-thaw
freeze-thaw

What repeated freezing does to a peptide in solution: ice-crystal shear at the growing front, freeze-concentration of solutes, pH shifts as buffer…

55 questions
peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
storage
storage

Storage conditions and their evidence base: minus twenty degrees for powder, refrigerated for solution, protection from light, and what the…

646 questions
asked by v_ramaswamy 40k · 2.3 years ago
21 votes 5 answers5.4k views
How many freeze-thaw cycles will survodutide at 8 mg/mL tolerate?

Conditions: survodutide · 8 mg/mL. I am trying to do this correctly the first time rather than learn it by getting it wrong. I have already made one mistake here that…

freeze-thaw
freeze-thaw

What repeated freezing does to a peptide in solution: ice-crystal shear at the growing front, freeze-concentration of solutes, pH shifts as buffer…

55 questions
peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
storage
storage

Storage conditions and their evidence base: minus twenty degrees for powder, refrigerated for solution, protection from light, and what the…

646 questions
asked by yuki_morishita 19k · 8 days ago
20 votes 5 answers22k views
How many freeze-thaw cycles will oral semaglutide at 1 mg/mL tolerate?

The specifics, since they change the answer: oral semaglutide · 1 mg/mL. I want a method I can write down and repeat, not a rule of thumb. I would rather…

freeze-thaw
freeze-thaw

What repeated freezing does to a peptide in solution: ice-crystal shear at the growing front, freeze-concentration of solutes, pH shifts as buffer…

55 questions
peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
storage
storage

Storage conditions and their evidence base: minus twenty degrees for powder, refrigerated for solution, protection from light, and what the…

646 questions
asked by tare_weight 47k · 17 months ago
19 votes 5 answers30k views
How many freeze-thaw cycles will tirzepatide at 1 mg/mL tolerate?

For reference: tirzepatide · 1 mg/mL. I want a method I can write down and repeat, not a rule of thumb. I would rather over-engineer this than discover a problem…

freeze-thaw
freeze-thaw

What repeated freezing does to a peptide in solution: ice-crystal shear at the growing front, freeze-concentration of solutes, pH shifts as buffer…

55 questions
peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
storage
storage

Storage conditions and their evidence base: minus twenty degrees for powder, refrigerated for solution, protection from light, and what the…

646 questions
asked by void_volume 13k · 2.2 years ago
19 votes3 answers16k views
What are mannitol and trehalose doing in the lyophilisation cake, and does their presence change how much freeze-thaw abuse a vial tolerates?

I have two vials of nominally the same compound from different suppliers. One cake is a dense white puck that sits proud in the vial; the other is a thin, almost…

lyophilisation
lyophilisation

Freeze-drying: primary and secondary drying, collapse temperature, the role of bulking agents and cryoprotectants such as mannitol and trehalose,…

17 questions
freeze-thaw
freeze-thaw

What repeated freezing does to a peptide in solution: ice-crystal shear at the growing front, freeze-concentration of solutes, pH shifts as buffer…

55 questions
peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
vial-inspection
vial-inspection

What you can learn by looking: cake morphology, meniscus films, fibres versus stopper fragments versus true particulates, clarity after…

66 questions
asked by ekaterina_volk 16k · 6 months ago
19 votes 5 answers10k views
How many freeze-thaw cycles will dulaglutide at 8 mg/mL tolerate?

Stated plainly: dulaglutide · 8 mg/mL. Everything I have found on this is either a forum aside or a product page, neither of which I trust. I am comfortable with the…

freeze-thaw
freeze-thaw

What repeated freezing does to a peptide in solution: ice-crystal shear at the growing front, freeze-concentration of solutes, pH shifts as buffer…

55 questions
peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
storage
storage

Storage conditions and their evidence base: minus twenty degrees for powder, refrigerated for solution, protection from light, and what the…

646 questions
asked by rota_site 55k · 13 months ago
19 votes0 answers5.1k views
How many freeze-thaw cycles will a GLP-1 receptor agonist at 6.67 mg/mL tolerate?

Details up front: a GLP-1 receptor agonist · 6.67 mg/mL. I would like to understand the steps well enough to explain them to someone else. I have access to a…

freeze-thaw
freeze-thaw

What repeated freezing does to a peptide in solution: ice-crystal shear at the growing front, freeze-concentration of solutes, pH shifts as buffer…

55 questions
peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
storage
storage

Storage conditions and their evidence base: minus twenty degrees for powder, refrigerated for solution, protection from light, and what the…

646 questions
asked by juan_esquivel 14k · 15 days ago
17 votes5 answers29k views
How many freeze-thaw cycles will oral semaglutide at 4 mg/mL tolerate?

The particulars: oral semaglutide · 4 mg/mL. I have read the obvious sources and they disagree with each other, so I would rather ask people who have actually done…

freeze-thaw
freeze-thaw

What repeated freezing does to a peptide in solution: ice-crystal shear at the growing front, freeze-concentration of solutes, pH shifts as buffer…

55 questions
peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
storage
storage

Storage conditions and their evidence base: minus twenty degrees for powder, refrigerated for solution, protection from light, and what the…

646 questions
asked by swab_and_wait 15k · 2.1 years ago
17 votes 5 answers7.1k views
How many freeze-thaw cycles will tirzepatide at 2.5 mg/mL tolerate?

The particulars: tirzepatide · 2.5 mg/mL. I have read the obvious sources and they disagree with each other, so I would rather ask people who have actually done this.…

freeze-thaw
freeze-thaw

What repeated freezing does to a peptide in solution: ice-crystal shear at the growing front, freeze-concentration of solutes, pH shifts as buffer…

55 questions
peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
storage
storage

Storage conditions and their evidence base: minus twenty degrees for powder, refrigerated for solution, protection from light, and what the…

646 questions
asked by rota_site 55k · 6 months ago
16 votes 5 answers10k views
How many freeze-thaw cycles will liraglutide at 2.5 mg/mL tolerate?

The particulars: liraglutide · 2.5 mg/mL. I want a method I can write down and repeat, not a rule of thumb. I would rather over-engineer this than discover a problem…

freeze-thaw
freeze-thaw

What repeated freezing does to a peptide in solution: ice-crystal shear at the growing front, freeze-concentration of solutes, pH shifts as buffer…

55 questions
peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
storage
storage

Storage conditions and their evidence base: minus twenty degrees for powder, refrigerated for solution, protection from light, and what the…

646 questions
asked by unit_math 13k · 7 months ago
16 votes 5 answers3.1k views
How many freeze-thaw cycles will ecnoglutide at 20 mg/mL tolerate?

The case in front of me: ecnoglutide · 20 mg/mL. I am trying to do this correctly the first time rather than learn it by getting it wrong. I have already made one…

freeze-thaw
freeze-thaw

What repeated freezing does to a peptide in solution: ice-crystal shear at the growing front, freeze-concentration of solutes, pH shifts as buffer…

55 questions
peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
storage
storage

Storage conditions and their evidence base: minus twenty degrees for powder, refrigerated for solution, protection from light, and what the…

646 questions
asked by anouk_desmet 18k · 2 months ago
14 votes5 answers14k views
How many freeze-thaw cycles will semaglutide at 8 mg/mL tolerate?

Concretely: semaglutide · 8 mg/mL. I have done this once and I suspect I got away with it rather than got it right. For context: I keep records of every batch, every…

freeze-thaw
freeze-thaw

What repeated freezing does to a peptide in solution: ice-crystal shear at the growing front, freeze-concentration of solutes, pH shifts as buffer…

55 questions
peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
storage
storage

Storage conditions and their evidence base: minus twenty degrees for powder, refrigerated for solution, protection from light, and what the…

646 questions
asked by leah_ferrers 15k · 7 months ago
12 votes 5 answers27k views
How many freeze-thaw cycles will a GLP-1 receptor agonist at 1 mg/mL tolerate?

What I have: a GLP-1 receptor agonist · 1 mg/mL. I can find plenty of assertions about this and almost no reasoning, which is usually a sign that nobody has checked.…

freeze-thaw
freeze-thaw

What repeated freezing does to a peptide in solution: ice-crystal shear at the growing front, freeze-concentration of solutes, pH shifts as buffer…

55 questions
peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
storage
storage

Storage conditions and their evidence base: minus twenty degrees for powder, refrigerated for solution, protection from light, and what the…

646 questions
asked by per_haugen 18k · 23 months ago
12 votes 4 answers25k views
How many freeze-thaw cycles will liraglutide at 1 mg/mL tolerate?

The specifics, since they change the answer: liraglutide · 1 mg/mL. I have read the obvious sources and they disagree with each other, so I would rather ask people…

freeze-thaw
freeze-thaw

What repeated freezing does to a peptide in solution: ice-crystal shear at the growing front, freeze-concentration of solutes, pH shifts as buffer…

55 questions
peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
storage
storage

Storage conditions and their evidence base: minus twenty degrees for powder, refrigerated for solution, protection from light, and what the…

646 questions
asked by thermal_mass 16k · 2.0 years ago
12 votes5 answers15k views
Why does freeze-concentration shift pH during freezing?

Transit was eleven days with two scanning gaps, so the thermal history is partly inferred. I would like the mechanism, because I want to be able to reason about the…

freeze-thaw
freeze-thaw

What repeated freezing does to a peptide in solution: ice-crystal shear at the growing front, freeze-concentration of solutes, pH shifts as buffer…

55 questions
peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
lyophilisation
lyophilisation

Freeze-drying: primary and secondary drying, collapse temperature, the role of bulking agents and cryoprotectants such as mannitol and trehalose,…

17 questions
asked by s_kalniete 47k · 2.4 years ago
12 votes5 answers11k views
How many freeze-thaw cycles will mazdutide at 6.67 mg/mL tolerate?

Numbers first: mazdutide · 6.67 mg/mL. I can find plenty of assertions about this and almost no reasoning, which is usually a sign that nobody has checked. Assume no…

freeze-thaw
freeze-thaw

What repeated freezing does to a peptide in solution: ice-crystal shear at the growing front, freeze-concentration of solutes, pH shifts as buffer…

55 questions
peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
storage
storage

Storage conditions and their evidence base: minus twenty degrees for powder, refrigerated for solution, protection from light, and what the…

646 questions
asked by anja_hellstrom 13k · 4 months ago
12 votes0 answers3.4k views
Can I re-freeze a lyophilised vial that reached room temperature in transit?

This is a recurring problem with one particular shipping lane rather than a one-off. Everything I have found on this is either a forum aside or a product page,…

freeze-thaw
freeze-thaw

What repeated freezing does to a peptide in solution: ice-crystal shear at the growing front, freeze-concentration of solutes, pH shifts as buffer…

55 questions
storage
storage

Storage conditions and their evidence base: minus twenty degrees for powder, refrigerated for solution, protection from light, and what the…

646 questions
cold-chain
cold-chain

Keeping material within a temperature window from manufacture to use: phase-change packs versus dry ice, thermal mass, transit-lane temperature…

524 questions
asked by lucia_marchetti 18k · 11 months ago
11 votes 4 answers20k views
How many freeze-thaw cycles will orforglipron at 8 mg/mL tolerate?

Stated plainly: orforglipron · 8 mg/mL. Everything I have found on this is either a forum aside or a product page, neither of which I trust. I am comfortable with the…

freeze-thaw
freeze-thaw

What repeated freezing does to a peptide in solution: ice-crystal shear at the growing front, freeze-concentration of solutes, pH shifts as buffer…

55 questions
peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
storage
storage

Storage conditions and their evidence base: minus twenty degrees for powder, refrigerated for solution, protection from light, and what the…

646 questions
asked by nynke_dekker 18k · 2.0 years ago
10 votes5 answers5.9k views
How many freeze-thaw cycles will dulaglutide at 20 mg/mL tolerate?

Stated plainly: dulaglutide · 20 mg/mL. This is a procedural question rather than a theoretical one, and I would like the procedure rather than the theory. What I…

freeze-thaw
freeze-thaw

What repeated freezing does to a peptide in solution: ice-crystal shear at the growing front, freeze-concentration of solutes, pH shifts as buffer…

55 questions
peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
storage
storage

Storage conditions and their evidence base: minus twenty degrees for powder, refrigerated for solution, protection from light, and what the…

646 questions
asked by nadia_kowalczyk 14k · 10 months ago
8 votes 5 answers26k views
How many freeze-thaw cycles will retatrutide at 2 mg/mL tolerate?

Stated plainly: retatrutide · 2 mg/mL. Everything I have found on this is either a forum aside or a product page, neither of which I trust. I am comfortable with the…

freeze-thaw
freeze-thaw

What repeated freezing does to a peptide in solution: ice-crystal shear at the growing front, freeze-concentration of solutes, pH shifts as buffer…

55 questions
peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
storage
storage

Storage conditions and their evidence base: minus twenty degrees for powder, refrigerated for solution, protection from light, and what the…

646 questions
asked by Dr_Fatima_Belkacem 52k · 23 months ago
8 votes5 answers7.9k views
How many freeze-thaw cycles will survodutide at 2 mg/mL tolerate?

The case in front of me: survodutide · 2 mg/mL. I have done this once and I suspect I got away with it rather than got it right. For context: I keep records of every…

freeze-thaw
freeze-thaw

What repeated freezing does to a peptide in solution: ice-crystal shear at the growing front, freeze-concentration of solutes, pH shifts as buffer…

55 questions
peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
storage
storage

Storage conditions and their evidence base: minus twenty degrees for powder, refrigerated for solution, protection from light, and what the…

646 questions
asked by shear_at_the_front 15k · 23 months ago
7 votes 5 answers20k views
How many freeze-thaw cycles will dulaglutide at 5 mg/mL tolerate?

What I am working with: dulaglutide · 5 mg/mL. This is a procedural question rather than a theoretical one, and I would like the procedure rather than the theory.…

freeze-thaw
freeze-thaw

What repeated freezing does to a peptide in solution: ice-crystal shear at the growing front, freeze-concentration of solutes, pH shifts as buffer…

55 questions
peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
storage
storage

Storage conditions and their evidence base: minus twenty degrees for powder, refrigerated for solution, protection from light, and what the…

646 questions
asked by nine_point_nine 45k · 17 months ago