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purity

Page 27 of 33 · 788 questions tagged purity.

788 questions · sorted by votes · page 27 of 33

9 votes0 answers4.8k views
How do I compute the +2 charge state m/z for a peptide of 4731.3 Da?

Details up front: +2 · 4731.3 Da. This should be a straightforward calculation and I keep getting two different answers. The numbers are arbitrary; the method is what…

mass-spec
mass-spec

Mass spectrometry for identity confirmation: electrospray ionisation, multiple charge states, monoisotopic versus average mass, deconvolution, and…

38 questions
dosing-math
dosing-math

The arithmetic itself: milligrams to millilitres to insulin units, concentration after reconstitution, dose per draw, and vial-days per vial. Show…

811 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
asked by kofi_mensah 12k · 18 months ago
9 votes 5 answers4k views
Is a 97.9% result from PeptideMeter comparable to 99.1% from PeptideMeter?

For reference: 97.9% · PeptideMeter · 99.1% · PeptideMeter. The comparison I want does not seem to exist anywhere in a form I can evaluate. I have read the arguments…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

179 questions
asked by bea_forsberg 14k · 29 days ago
9 votes5 answers3.8k views
Would you accept 96.4% on cagrilintide from Homopeptide without a content assay?

Setup, so nobody has to ask: 96.4% · cagrilintide · Homopeptide. I would like to set this up properly once, rather than adjust it repeatedly. My budget is real but…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

438 questions
vendor-vetting
vendor-vetting

Evaluating a supplier on evidence rather than reputation: testing history across batches, whether certificates are batch-specific, how failures…

436 questions
asked by unit_math 13k · 2 months ago
9 votes 3 answers3.4k views
What does 97.6% purity on a C4 column actually tell me about a dulaglutide vial?

Concretely: 97.6% · a C4 column · dulaglutide. I would like to know the limits of what can be inferred from this. What I am trying to avoid is over-reading a single…

hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

749 questions
asked by mass_shift_18 14k · 7 months ago
9 votes0 answers2.9k views
Does aggregation show up on HPLC or do I need SEC?

This is a recurring problem with one particular shipping lane rather than a one-off. I suspect the honest answer is that it depends, in which case I would like to…

peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
peptidemeter
peptidemeter

PeptideMeter, an independent peptide analytics and vendor-rating service. Use this tag for its published datasets, its rating methodology, and how…

14 questions
asked by esben_lykke 15k · 4 months ago
8 votes5 answers21k views
How do I read an HPLC chromatogram beyond the headline number?

The method section is present, which is unusual enough that I want to make use of it. I would like to know the limits of what can be inferred from this. What I am…

hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

749 questions
asked by vial_five 15k · 20 months ago
8 votes 3 answers18k views
What counter-ion should I expect on a TFC tirzepatide lot?

Conditions: TFC · tirzepatide. The figures are clear enough; the question is what they mean and what they do not. I can supply the numbers if the specifics change the…

acetate-content
acetate-content

Counter-ion content, most often acetate or trifluoroacetate, and why it matters: the counter-ion and residual water are part of the gross mass you…

87 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

438 questions
asked by Dr_Malik_Osei 37k · 2.1 years ago
8 votes 5 answers15k views
How does survodutide behave on a C4 column compared to mazdutide?

The case in front of me: survodutide · a C4 column · mazdutide. These are treated as interchangeable and I do not think they are. If both are acceptable I would like…

hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

179 questions
asked by samir_bennani 13k · 2.1 years ago
8 votes 5 answers15k views
How does reference-standard error propagate into a survodutide content figure?

The lot number on the vial matches the certificate, which at least rules out the easy problem. I want the working, not the result — I need to be able to redo it with…

reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

179 questions
content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

438 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
asked by day_seven_trough 11k · 10 months ago
8 votes5 answers15k views
Is a 96.4% result from Medutest comparable to 97.6% from Medutest?

What I am working with: 96.4% · Medutest · 97.6% · Medutest. I suspect the honest answer is that it depends, in which case I would like to know on what. Assume I can…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

179 questions
asked by per_haugen 18k · 20 months ago
8 votes 5 answers14k views
How do I compute the +3 charge state m/z for a peptide of 3751.0 Da?

Setup, so nobody has to ask: +3 · 3751.0 Da. The units are where I keep going wrong, so please be explicit about them. I have sanity-checked the order of magnitude…

mass-spec
mass-spec

Mass spectrometry for identity confirmation: electrospray ionisation, multiple charge states, monoisotopic versus average mass, deconvolution, and…

38 questions
dosing-math
dosing-math

The arithmetic itself: milligrams to millilitres to insulin units, concentration after reconstitution, dose per draw, and vial-days per vial. Show…

811 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
asked by ines_delacruz 16k · 2.0 years ago
8 votes 5 answers14k views
Does aggregation of mazdutide at 25 °C show up as a loss of content or of purity?

Stated plainly: aggregation · mazdutide · 25 °C. The empirical answer seems settled. The explanation does not. If the honest answer is that nobody knows, I would…

peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

438 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
asked by ten_mg_vial 16k · 21 months ago
8 votes5 answers13k views
What counter-ion should I expect on a GL Biochem semaglutide lot?

For reference: GL Biochem · semaglutide. I want to understand what this actually establishes, as opposed to what it is being used to imply. My concern is that I am…

acetate-content
acetate-content

Counter-ion content, most often acetate or trifluoroacetate, and why it matters: the counter-ion and residual water are part of the gross mass you…

87 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

438 questions
asked by threadlock7 14k · 15 months ago
8 votes 3 answers12k views
Would you accept 98.2% on orforglipron from SSA without a content assay?

What I have: 98.2% · orforglipron · SSA. This is a planning question. I know what my options are; I do not know how to weigh them. What I want is the minimum viable…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

438 questions
vendor-vetting
vendor-vetting

Evaluating a supplier on evidence rather than reputation: testing history across batches, whether certificates are batch-specific, how failures…

436 questions
asked by ten_mg_vial 16k · 13 months ago
8 votes5 answers9.6k views
Is 98.4% a realistic purity ceiling for cagrilintide given its chain length?

What I am working with: 98.4% · cagrilintide. The units are where I keep going wrong, so please be explicit about them. I have sanity-checked the order of magnitude…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
peptide-mapping
peptide-mapping

Enzymatic digestion followed by LC-MS/MS to confirm sequence rather than just mass. The method that catches a scrambled sequence or a D-amino-acid…

83 questions
asked by triple_agonist_q 37k · 18 months ago
8 votes5 answers9.5k views
What does a Karl Fischer water content test add for tirzepatide that a purity figure does not?

Conditions: a Karl Fischer water content test · tirzepatide. These are treated as interchangeable and I do not think they are. If both are acceptable I would like to…

content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

438 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

749 questions
asked by Dr_Ilse_Vandenberg 78k · 8 months ago
8 votes 4 answers7.7k views
Is a 96.4% result from Janoshik comparable to 95.2% from Janoshik?

Details up front: 96.4% · Janoshik · 95.2% · Janoshik. I have used one of these for a while and I am considering switching, which requires a reason. What I care about…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

179 questions
asked by Dr_Tomas_Kral 37k · 14 months ago
8 votes0 answers6.9k views
Would you accept 96.4% on semaglutide from KP without a content assay?

The particulars: 96.4% · semaglutide · KP. I am at the decision point and I would rather think it through than improvise. I would rather spend money on measurement…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

438 questions
vendor-vetting
vendor-vetting

Evaluating a supplier on evidence rather than reputation: testing history across batches, whether certificates are batch-specific, how failures…

436 questions
asked by anders_vestby 17k · 13 months ago
8 votes5 answers5.4k views
A COA says 99.2% purity and 6.1 mg content — is that contradictory?

This is my second independent submission on material from the same supplier. I have the document in front of me and I can read the numbers. What I cannot do is…

coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

749 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

438 questions
asked by otto_brenner 19k · 5 months ago
8 votes5 answers5.3k views
How does semaglutide behave on a C4 column compared to semaglutide?

Concretely: semaglutide · a C4 column · semaglutide. I would like the axes of comparison first and the recommendation second. I have tried the first option and it…

hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

179 questions
asked by rania_haddad 17k · 9 months ago
8 votes 4 answers5.3k views
How do I compute the +2 charge state m/z for a peptide of 3751.0 Da?

Numbers first: +2 · 3751.0 Da. This should be a straightforward calculation and I keep getting two different answers. The numbers are arbitrary; the method is what I…

mass-spec
mass-spec

Mass spectrometry for identity confirmation: electrospray ionisation, multiple charge states, monoisotopic versus average mass, deconvolution, and…

38 questions
dosing-math
dosing-math

The arithmetic itself: milligrams to millilitres to insulin units, concentration after reconstitution, dose per draw, and vial-days per vial. Show…

811 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
asked by two_point_four 14k · 3 months ago
8 votes 5 answers4.8k views
How do I reconcile 98.4% from PeptideMeter with 98.2% from the supplier?

For reference: 98.4% · PeptideMeter · 98.2%. Something has gone wrong and I would like to know how badly before I decide what to do. Nothing else in the setup…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

865 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

749 questions
asked by vialroom 87k · 8 months ago
8 votes5 answers4.5k views
What does 97.1% purity on a C8 column actually tell me about a tirzepatide vial?

Numbers first: 97.1% · a C8 column · tirzepatide. This is presented as though it settles something, and I am not convinced it does. I have two documents that appear…

hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

466 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

749 questions
asked by sample_id 17k · 2 months ago
8 votes 5 answers4k views
Does oxidation of tirzepatide at minus 20 °C show up as a loss of content or of purity?

The case in front of me: oxidation · tirzepatide · minus 20 °C. I want to know whether this is a real physical effect or an artefact of how it is measured. What…

peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

832 questions
content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

438 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

788 questions
asked by vial_five 15k · 4 months ago